Benedict's solution is a deep-blue alkaline solution used to test for the presence of the aldehyde functional group, -CHO. The substance to be tested is heated with Benedict's solution; formation of a brick-red precipitate indicates presence of the aldehyde group. Since simple sugars (e.g., glucose) give a positive test, the solution is used to test for the presence of glucose in urine, a symptom of diabetes. One liter of Benedict's solution contains 173 grams sodium citrate, 100 grams sodium carbonate, and 17.3 grams cupric sulfate pentahydrate. When heated with reducing sugar, the cupric ion (complexed with citrate ions) is reduced to cuprous ion by the aldehyde group (which is oxidized), and precipitates as cuprous oxide, Cu2O.
One-liter material set:
The one-liter material set contains 3 parts in three different plastic bags as follows.
Part 1: 17.3 grams cupric sulfate pentahydrate
Part 2: 173 grams sodium citrate
Part 3: 100 grams sodium carbonate
How to prepare the solution?
This set contain hazardous materials. Keep them away from small children. Avoid skin contact and contact a physician if accidentally ingested.