Bacteria Growth Science Set
In a bacteria growth science set you will find the following items:
You can use the material in this kit to experiments on collecting and growing household bacteria. When you grow bacteria, you will then be able to go further and research on bacteria and factors affecting their growth and reproduction.
Gather information about your project. Read books, magazines or ask professionals who might know in order to learn more about the subject of your research.
Bacteria are single-celled micro organisms that play an important role in many ecosystems. Bacteria vary in terms of morphology, oxygen and nutritional requirements and motility. Bacteria may be free-living, live on dead or decaying organic matter (saprophytic), or pathogenic in plants or animals. Bacteria reproduce by fission.
to prepare a culture media?
In order to grow bacteria, you will need culture media, plates or petri-dishes and some laboratory supplies and an incubator.
Culture Media: Culture media is a moist or liquid matter that contains nutrients for bacteria. Almost any nutrient food may be considered a culture media for general bacteria, however if you want to grow a specific bacteria or prevent growing some other bacteria, you will need to use a fine tuned recipe for your culture media.
Chicken broth and beef broth are among nutrients that most bacteria can use. In some recipes you may also add some mushroom extract. Sugar can also be added to some culture media. Small amounts of some minerals such as potassium phosphate and calcium carbonate may also be added to the culture media. Note that there are many foods that are good for growing bacteria, but they are not good as culture media. For example bacteria can easily grow on milk, but milk is not a good culture media because it will change by the activity of bacteria. Part of milk solidify when bacteria produce acids. A good culture media must be clear and must remain liquid and should not easily change pH. If you need to solidify your culture media, use agar to do that. Agar is a gelatinous substance that is extracted from sea weeds. If we need to grow bacteria for the purpose of identification or counting, we need to grow bacteria in nutrient agar plates. These are petri-dishes containing a mixture of agar and nutrients.
Incubator: Incubator is a warm cabinet that you can set its temperature to a proper temperature for bacteria growth. About 35º C is a good temperature for most bacteria. This is close to the body temperature. If you are able to create such a temperature in any other way, it is as good as an incubator. You may find warm places behind your refrigerator, next to the radiator or inside an oven that is off.
You may also make an incubator by placing a small desk lamp inside a wooden or metal box. Or you may put a Styrofoam cooler upside down over a desk lamp. A small lamp (15 watts) should be able to create enough heat to warm up a small space. Prepare your incubator in advance and use a thermometer to test it a day before starting your experiment.
* Chicken broth or beef broth can be purchased from supermarkets and health food stores or you may make them at home. (It must be fat free). Filter paper is coffee filter or you may substitute it with any clean cotton cloth.
How to prepare nutrient agar plates?
Prepare the nutrients such as chicken broth or beef broth and boil it with some mushroom. Let it cool off to the room temperature and filter it to be clear. Add water to bring it to 800ml to 1000 ml. Add 7 to 10 grams of agar and stir it to make an emulsion.
Reheat the emotion while stirring until it boils for about one minute. Your nutrient agar is now ready. Let it cool to about 45ºC. At this time you can pour the nutrient agar solution in petri-dishes and place the lid. The amount of nutrient agar in each plate must be enough to cover the bottom of the plate. Agar will solidify in plates.
Note that boiling does not kill all the bacteria and your agar and your plates may not be sterile at this time. If you have access to an autoclave, place your plates in autoclave for about one hour at the temperature of 135ºC. Without autoclave your results will not be reliable and some unexpected bacteria may grow in your nutrient agar.
You can keep your nutrient agar plates in a refrigerator for later use. When you are ready to use a plate, remove it from the refrigerator and keep it in room temperature for a few hours.
To grow bacteria, use a pipette to drop 0.1 ml of infected water or any other sample on one agar plate and spread it on the surface of the agar using an sterile glass rod or spoon. Place the plate in an incubator or any other place with a temperature of about 35ºC. Within 24 to 48 hours you should be able to observe bacteria colonies.
This page contains some general information for bacteria growth experiments. You need to refer to more scientific resources for more accurate and up to date information. This page may be updated from time to time.